陈雯;王静捷;黄宇光;
CHEN Wen,WANG Jing-jie,HUANG Yu-guang Department of Anesthesiology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

• 1:中国医学科学院北京协和医学院北京协和医院麻醉科

摘要(Abstract)：

目的探讨用Walker 256大鼠乳腺癌细胞建立大鼠胫骨癌痛模型的可行性。方法将36只SD雌性大鼠,随机分为肿瘤组(n=8)、假手术1(n=10)、假手术2(n=10)和正常对照组(n=8)。将含1×105 Walker 256大鼠乳腺癌细胞悬液注入大鼠胫骨上段制备骨癌痛模型,观察疼痛行为学[包括机械缩足反射阈值(mechanical paw with-drawal threshold,MWT)和热缩足反应潜伏期(paw withdrawal thermal latency,PWTL)]、骨放射学和组织学变化。结果疼痛行为学、影像学、组织学观察结果证实,Walker 256大鼠乳腺癌细胞能成功制备大鼠胫骨癌痛模型。肿瘤组造模后第7天开始MWT显著降低,为(37.59±2.02)g,与假手术组1、2和正常对照组比较,P<0.01;第11天PWTL显著降低,为(6.87±1.00)s,与假手术组1、2和正常对照组比较,P<0.01。结论用Walker 256大鼠乳腺癌细胞能够成功建立大鼠胫骨癌痛模型。
Objective To explore the feasibility of establishing a rat model of tibial cancer pain with Walker 256 mammary gland carcinoma cells.Methods Totally 36 female SD rats were randomly divided into 4 groups:Sarcoma group(n=8),Sham 1 group(n=10);Sham 2 group(n=10);and Nave group(n=8).In each treatment group,105 syngenenic Walker 256 mammary gland carcinoma cells,ascetic fluid without tumor cells,and normal saline were respectively injected into the tibia medullary cavity via intercondylar eminence.The pain behaviors including mechanical paw withdrawal threshold(MWT) and paw withdrawal thermal latency(PWTL),bone radiology,and bone histology were evaluated.Results Observational results via pain behaviors,radiology,and histology demonstrated that rat model of tibial cancer pain was successfully established with Walker 256 mammary gland carcinoma cells.MWT significantly decreased to 37.59±2.02g on the 7th day after tumor cell injection in the Sarcoma group(P<0.01 compared with Sham 1 group,Sham 2 group,and Nave group).PWTL significantly decreased to 6.87±1.00s on the 11th day(P<0.01 compared with Sham 1 group,Sham 2 group,and Nave group).Conclusion Rat model of tibial cancer pain can be successfully established with Walker 256 mammary gland carcinoma cells.

关键词(KeyWords)： Walker 256肿瘤细胞;骨癌痛模型
Walker 256 tumor cell;bone cancer pain model

Abstract:

Keywords:

基金项目(Foundation): 中央保健专项基金(B2009B076)

作者(Authors): 陈雯;王静捷;黄宇光;
CHEN Wen,WANG Jing-jie,HUANG Yu-guang Department of Anesthesiology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

参考文献(References)：

• [1]Paice JA,Ferrell B.The management of cancer pain[J].Ca Cancer J Clin,2011,61:157-182.
• [2]Meuser T,Pietruck C,Radbruch L,et al.Symptoms during cancer pain treatment following WHO guidelines:a longitudinal follow up study of symptom prevalence,severity and etiology[J].Pain,2001,93:247-257.
• [3]Coleman RE.Skeletal complications of malignancy[J].Cancer,1997,80(8 Suppl):1588-1594.
• [4]Thapa D,Rastogi V,Ahuja V.Cancer pain managementcurrent status[J].J Anaesthesiol Clin Pharmacol,2011,27:162-168.
• [5]Gralow JR,Biermann JS,Farooki A,et al.NCCN task force report:bone health in cancer care[J].J Natl Compr Canc Netw,2009,7(Suppl3):S1-S35.
• [6]Mercadante S,Arcuri E.Breakthrough pain in cancer patients:pathophysiology and treatment[J].Cancer Treat Rev,1998,24:425-432.
• [7]Mercadante S.Malignant bone pain:pathophysiology and treatment[J].Pain,1997,69:1-18.
• [8]Lipton A,Uzzo R,Amato RJ,et al.The science and practice of bone health in oncology:managing bone loss and metastasis in patient with solid tumors[J].J Natl Compr Canc Netw,2009,7(Suppl7):S1-S30.
• [9]Portenoy RK,Payne D,Jacobsen P.Breakthrough pain:characteristics and impact in patients with cancer pain[J].Pain,1999,81:129-134.
• [10]Luger,NM,Honore P,Sabino MAC,et al.Osteoprotegerin diminishes advanced bone cancer pain[J].Cancer Res,2001,61:4038-4047.
• [11]Wacnik PW,Kehl LJ,Trempe TM,et al.Tumor implantation in mouse humerus evokes movement-related hyperalgesia exceeding that evoked by intramuscular carrageenan[J].Pain,2003,101:175-186.
• [12]Pacharinsak C,Beitz A.Animal models of cancer pain[J].Comp Med,2008,58:220-233.
• [13]Medhurst SJ,Walker K,Bowes M,et al.A rat model of bone cancer pain[J].Pain,2002,96:129-140.
• [14]Vivancos GG,Verri WA Jr,Cunha TM,et al.An electronic pressure-meter nociception paw test for rats[J].Braz J Med Biol Res,2004,37:391-399.
• [15]Dornelas CA,Almeida PR,Nascimento GL,et al.Experimental model of Walker 256 carcinosarcoma in rats bladder[J].Acta Cir Bras,2006,21:38-42.
• [16]Buffon A,Ribeiro VB,Schanoki AS,et al.Diminution in adenine nucleotide hydrolysis by platelets and serum from rats submitted to Walker 256 tumour[J].Mol Cell Biochem,2006,281:189-195.