协和医学杂志

2013, (03)

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内毒素休克小鼠脂钙蛋白2及其相关细胞因子的表达
The Expression of Lipocalin 2 and Its Relative Cytokines in Endotoxic mice

曾皋;郭树彬

摘要(Abstract):

摘要:目的 探讨内毒素对小鼠脂钙蛋白2及其相关细胞因子表达水平的影响。方法 C57BL/6小鼠予内毒素腹腔内注射,10mg/kg、20 mg/kg、30 mg/kg各为1组,对照组仅注射生理盐水(n=8)。观察24小时后留取盲肠和血清,ELISA和Western blot测定IL-1β、IL-6、IL-17A、LCN2,实时PCR相对定量肠组织中LCN2 mRNA。结果 与非造模组比较,大剂量内毒素组(20 mg /kg和30 mg /kg)的血清IL-6、LCN2与肠组织IL-1β、IL-6、IL-17A、LCN2显著增高。与10 mg /kg组比较,30 mg /kg组的血清IL-6、LCN2水平以及肠组织IL-17A、LCN2、IL-1β显著增高。各组2-ΔΔCT以均值±标准差表示,对照组1.548±0.417,10mg/kg 组3.184±0.716,20mg/kg组 4.875±0.547, 30mg/kg组7.489±0.820,各组两两比较均显示统计学差异。结论 内毒素剂量依赖性地引起小鼠肠组织LCN2表达水平增高;血清IL-6和 LCN2水平以及肠组织IL-1β、IL-17A与LCN2水平之间存在一定的相关性。
ABSTRACT: Objective To explore the influence of endotoxin (LPS) injection on the expression of lipocalin 2 and its relative cytokines in mice. Methods C57BL/6 mice were divided into 3 groups according to the serial LPS dosage of 10mg/kg, 20 mg/kg and 30 mg/kg (n=8). The control group received only isotonic saline injection. 24 hours later, cecum and blood were collected, and ELISA and Western blot were used to detect IL-1β、IL-17A、LCN2, real time PCR to examine the relative level of LCN2 mRNA in cecum. Results The levels of IL-6 and lCN2 in serum as well as the levels of IL17A, IL-1β, IL-6, LCN2 in cecum lysate in 2 large dosage LPS groups (20mg/kg & 30mg/kg) were significantly higher than their counterparts in sham model group. Compared with 10mg/kg LPS group, the levels of IL-6 and lCN2 in serum as well as the levels of IL-1β,IL17A, and LCN2 in cecum lysate increased statistically in the two large dosage LPS groups. 2-ΔΔCT was used to indicate fold changes of LCN2 mRNA in intestine. The results expressed as mean ± standard deviation are the following: 1.548±0.417 for control group, 3.184±0.716 for 10mg/kg group, 4.875±0.547 for 20mg/kg group, and 7.489±0.820 for 30mg/kg group. Conclusion LPS dose-dependently increased the level of LCN2 expression in cecum; Some kind of correlation exists between the level of IL-6 and lCN2 in serum as well as among the levels of IL17A, IL-1β, LCN2 in cecum.

关键词(KeyWords): |内毒素| 脂钙蛋白2|小鼠|
|endotoxin| lipocalin 2| mouse|

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作者(Author): 曾皋;郭树彬

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