协和医学杂志

2013, v.4(03) 260-264

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内毒素休克小鼠脂钙蛋白2及相关细胞因子的表达
Expressions of Lipocalin 2 and Its Relative Cytokines in Endotoxic Mice

曾皋;孙蓬然;刘安雷;刘洁;郭树彬;
ZENG Gao,SUN Peng-ran,LIU An-lei,LIU Jie,GUO Shu-bin Department of Emergency,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

摘要(Abstract):

目的探讨内毒素对小鼠脂钙蛋白2(lipocalin 2,LCN2)及其相关细胞因子表达水平的影响。方法 C57BL/6小鼠随机分为4组(n=8),实验组分别予10、20、30mg/kg内毒素腹腔内注射,对照组仅注射等容积生理盐水。观察24h后留取盲肠和血清,酶联免疫吸附法和蛋白免疫印迹法测定白细胞介素(interleukin,IL)-1β、IL-6、IL-17A、LCN2水平,实时PCR相对定量肠组织中LCN2 mRNA。结果与对照组比较,大剂量内毒素组(20mg/kg和30mg/kg)的血清IL-6、LCN2与肠组织IL-1β、IL-6、IL-17A、LCN2水平均显著增高(P<0.05)。与10mg/kg组比较,30mg/kg组的血清IL-6、LCN2以及肠组织IL-1β、IL-17A、LCN2水平均显著增高(P<0.05)。对照组及10、20、30mg/kg内毒素组肠组织LCN2mRNA相对定量值分别为1.548±0.417、3.184±0.716、4.875±0.547、7.489±0.820,两两比较差异均有统计学意义(P<0.05)。结论内毒素可剂量依赖性地引起小鼠肠组织LCN2表达水平增高;血清IL-6与LCN2水平以及肠组织IL-1β、IL-17A与LCN2水平之间存在一定的相关性。
Objective To explore the influence of an endotoxin lipopolysaccharide ( LPS) injection on the expressions of lipocalin 2 ( LCN2) and its relative cytokines in mice. Methods C57BL /6 mice were randomly divided into 3 groups ( n = 8 each) according to the serial LPS dosages of 10,20,and 30 mg / kg. The control group ( n = 8) received only isotonic saline injection. Twenty-four hours later,cecum and blood samples were collected,and enzyme-linked immunosorbent assay ( ELISA) and Western blot were performed to detect the serum levels of interleukin ( IL) -1β,IL-6,IL-17A,and LCN2,and real-time polymerase chain reaction ( PCR) to determine the LCN2 mRNA level in cecum lysate. Results The serum levels of IL-6 and LCN2 and the levels of IL-1β,IL-6,IL-17A,and LCN2 in cecum lysate in two large-dosage LPS groups ( 20 mg / kg and 30 mg / kg) were significantly higher than their counterparts in control group ( P < 0. 05) . Compared with the 10 mg / kg LPS group,the levels of IL-6 and LCN2 in serum and the levels of IL-1β,IL-17A,and LCN2 in cecum lysate significantly increased in the 30 mg / kg LPS group ( P < 0. 05 ) . 2 ΔΔCT was used to indicate fold changes of LCN2 mRNA in intestine. The results expressed as mean ± standard deviation were as follows: 1. 548 ± 0. 417 for control group,3. 184 ± 0. 716 for 10 mg / kg LPS group,4. 875 ± 0. 547 for 20 mg / kg LPS group,and 7. 489 ± 0. 820 for 30 mg / kg LPS group,and the difference was significant between either two groups ( P < 0. 05) . Conclusions LPS dose-dependently increases the level of LCN2 expression in cecum. The serum IL-6 level as well as the cecum IL-1β and IL-17A levels have certain correlations with the LCN2 level.

关键词(KeyWords): 内毒素;脂钙蛋白2;细胞因子
endotoxin; lipocalin 2; cytokine

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基金项目(Foundation): 黎介寿院士肠道屏障专项基金

作者(Author): 曾皋;孙蓬然;刘安雷;刘洁;郭树彬;
ZENG Gao,SUN Peng-ran,LIU An-lei,LIU Jie,GUO Shu-bin Department of Emergency,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

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