沈捷;明越;汤贤超;胡克;刘志凯;孙显松;李文博;杨志伟;姜菲;李楠;李利民;张福泉;
SHEN Jie;MING Yue;TANG Xian-chao;HU Ke;LIU Zhi-kai;SUN Xian-song;LI Wen-bo;YANG Zhi-wei;JIANG Fei;LI Nan;LI Li-min;ZHANG Fu-quan;Department of Radiation Oncology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Nuclear Medicine,Cancer Institute & Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Pathology,Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences & School

• 1:中国医学科学院 北京协和医学院 北京协和医院放射治疗科
• 2:中国医学科学院 北京协和医学院 肿瘤医院核医学科
• 3:中国医学科学院基础医学研究所
• 4:北京协和医学院基础学院病理系

摘要(Abstract)：

目的比较不同类型宫颈癌细胞株在不同剂量和剂量率射线照射下的放射敏感性特点。方法选取3种宫颈癌细胞株:CaSki、HeLa和SiHa细胞株,建立3种细胞放射敏感性模型,运用克隆形成实验分析不同剂量和剂量率照射下3种细胞的放射敏感性及其差别。结果 3种宫颈癌细胞放射敏感性参数如下:CaSki细胞在2 Gy照射后的存活分数为57.37%,HeLa细胞为70.62%,SiHa细胞为74.77%,三者差异具有统计学意义(P=0.002)。CaSki、HeLa、SiHa细胞在不同剂量率200、400、600 cGy/min照射后的存活分数差异无统计学意义(P>0.05)。结论 3种宫颈癌细胞的放射敏感性以CaSki为最高,HeLa次之,SiHa最低。本研究所用剂量率对3种细胞的存活分数无明显影响。
Objective To explore the radiosensitivity of different types of cervical cancer cells to different radiation doses and dose rates. Methods We established three cell radiosensitivity models with three cervical cancer cell lines: CaSki,HeLa,and SiHa. We then used clonogenic assay to analyze the radiosensitivity of these three cell lines to different radiation doses and dose rates. Results The survival fractions at 2 Gy(SF2) were57. 37%,70. 62%,and 74. 77% for CaSki,HeLa,and SiHa,respectively(P=0. 002). However,the survival fractions did not significantly differ when the three cell lines were treated with dose rates of 200,400,and 600 cGy / min(P>0. 05). Conclusions Among these three cell lines,CaSki has the highest radiosensitivity,followed by HeLa and SiHa. The dose rate used in our experiment showed no effect on the the survival fraction of three cervical cancer cell lines.

关键词(KeyWords)： 宫颈癌;放射敏感性;放射治疗
cervical cancer;radiosensitivity;radiotherapy

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81272891)

作者(Author): 沈捷;明越;汤贤超;胡克;刘志凯;孙显松;李文博;杨志伟;姜菲;李楠;李利民;张福泉;
SHEN Jie;MING Yue;TANG Xian-chao;HU Ke;LIU Zhi-kai;SUN Xian-song;LI Wen-bo;YANG Zhi-wei;JIANG Fei;LI Nan;LI Li-min;ZHANG Fu-quan;Department of Radiation Oncology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Nuclear Medicine,Cancer Institute & Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Pathology,Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences & School

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DOI:

参考文献(References)：

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