协和医学杂志

2012, v.3(04) 441-447

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筋脉通对糖尿病大鼠背根神经节氧化应激的影响
Effect of Jinmaitong on Oxidative Stress in Dorsal Root Ganglion of Diabetic Rats

张倩;梁晓春;孙青;王普艳;黄文智;李伯武;张彦东;高云周;
ZHANG Qian1,LIANG Xiao-chun1,SUN Qing1,WANG Pu-yan1,HUANG Wen-zhi1,LI Bo-wu1,ZHANG Yan-dong2,GAO Yun-zhou2 1Department of Traditional Chinese Medicine,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China 2Department of Pathology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

摘要(Abstract):

目的探讨中药复方筋脉通对糖尿病大鼠背根神经节尼氏染色及NADPH氧化酶、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的影响。方法 70只雄性Sprague Dawley大鼠腹腔注射链脲佐菌素诱导糖尿病大鼠模型后,随机分为模型组、筋脉通大、中、小剂量组和维生素C对照组,每组14只,同时设正常对照组(n=10);筋脉通大、中、小剂量组分别按成人剂量20倍、10倍、5倍、维生素C组按成人剂量10倍灌胃给药,模型组和正常对照组予等量蒸馏水灌胃;16周后对各组背根神经节切片行尼氏染色,免疫组化法检测NADPH氧化酶p22phox亚基和iNOS的表达。结果模型组神经元胞体中尼氏体缺失,筋脉通大、中剂量组尼氏体改变明显轻于模型组;筋脉通各剂量组NADPH氧化酶p22phox亚基、iNOS表达均明显低于模型组(P<0.05)。结论糖尿病大鼠周围感觉神经节存在氧化应激反应,筋脉通可减轻氧化应激损伤,保护神经元。
Objective To investigate the influence of Chinese compound formula Jinmaitong on the Nissl's staining of dorsal root ganglion (DRG) of diabetic rats and on the expressions of NADPH oxidase and inducible nitric oxide synthase (iNOS).Methods Dabetic rat models were induced after the intraperitoneal injection of streptozotocin (STZ) to 70 male Sprague Dawley rats.These rats were then divided into five groups (n=14):model group (placebo),Jinmaitong high-dose group (20-fold dose recommended for human),Jinmaitong middle-dose group (10-fold dose recommended for human),Jinmaitong low-dose group (5-fold dose recommened for human),and vitamin C group (10-fold dose recommened for human).Meanwhile,10 rats without STZ intervention were set as normal group.Drugs (or placebo) were intragastically administered once per day.Sixteen weeks later,DRG tissues were isolated for Nissl's staining as well as immunohistochemistry staining to detect the expressions of NADPH oxidase p22phox and iNOS.Results Nissl's bodies depleted obviously in model group.In Jinmaitong high-dose and middle-dose groups,the morphologic abnormality of Nissl's bodies was slight.Compared to model group,the expression of NADPH p22phox and iNOS in all Jinmaitong groups reduced significantly (P<0.05).Conclusions The study gives evidence that oxidative stress exists in peripheral sensory nerve ganglion of diabetic rats.Jinmaitong can counteract oxidative stress and protect neurons.

关键词(KeyWords): 活性氧;NADPH氧化酶;糖尿病;糖尿病周围神经病;氧化应激;一氧化氮合酶
reactive oxygen species;NADPH oxidase;diabetes mellitus;diabetic peripheral neuropathy;oxidative stress;nitric oxide synthase

Abstract:

Keywords:

基金项目(Foundation): 北京自然科学基金(7082077)

作者(Author): 张倩;梁晓春;孙青;王普艳;黄文智;李伯武;张彦东;高云周;
ZHANG Qian1,LIANG Xiao-chun1,SUN Qing1,WANG Pu-yan1,HUANG Wen-zhi1,LI Bo-wu1,ZHANG Yan-dong2,GAO Yun-zhou2 1Department of Traditional Chinese Medicine,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China 2Department of Pathology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

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